H2N2V1, Main, Exploration, bibRecord, 001354

Hemagglutinin pseudotyped lentiviral particles: characterization of a new method for avian H5N1 influenza sero-diagnosis.

Identifieur interne : 001354 ( Main/Exploration ); précédent : 001353; suivant : 001355

Hemagglutinin pseudotyped lentiviral particles: characterization of a new method for avian H5N1 influenza sero-diagnosis.

Auteurs : Isabelle Nefkens [Hong Kong] ; Jean-Michel Garcia [Hong Kong] ; Chu Shui Ling [Hong Kong] ; Nadège Lagarde [Hong Kong] ; John Nicholls [République populaire de Chine] ; Dong Jiang Tang [Hong Kong] ; Malik Peiris [Hong Kong] ; Philippe Buchy [Cambodge] ; Ralf Altmeyer [Hong Kong]

Source :

Journal of Clinical Virology [ 1386-6532 ] ; 2007-05.

RBID : Hal:pasteur-00589029

Abstract

BACKGROUND: Highly pathogenic avian influenza (HPAI) H5N1 has spread globally in birds and infected over 270 humans with an apparently high mortality rate. Serologic studies to determine the extent of asymptomatic H5N1 infection in humans and other mammals and to investigate the immunogenicity of current H5N1 vaccine candidates have been hampered by the biosafety requirements needed for H5N1 micro-neutralization tests. OBJECTIVE: Development of a serodiagnostic tool for highly pathogenic influenza that reproduces H5N1 biology but can be used with less biohazard. STUDY DESIGN: We have generated and evaluated H5 hemagglutinin pseudotyped lentiviral particles encoding the luciferase reporter (H5pp). RESULTS: H5pp entry into target cells depends on alpha2-3 cell surface sialic acids and requires low pH for membrane fusion. H5pp infectivity is specifically neutralized by sera from patients and animals infected with H5N1 and correlates well with conventional microneutralization test. CONCLUSIONS: H5pp reproduce H5N1 influenza virus entry into target cells and potentially provides a high-throughput and safe method for sero-epidemiology.

Url:

https://hal-riip.archives-ouvertes.fr/pasteur-00589029

DOI: 10.1016/j.jcv.2007.02.005

Affiliations:

Cambodge, Hong Kong, République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000125
to stream Hal, to step Curation: 000125
to stream Hal, to step Checkpoint: 000318
to stream Main, to step Merge: 001367
to stream Main, to step Curation: 001354

Le document en format XML

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            <ref type="url">https://research.pasteur.fr/ip-network</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Cambodge</country>
</affiliation>
</author>
<author><name sortKey="Altmeyer, Ralf" sort="Altmeyer, Ralf" uniqKey="Altmeyer R" first="Ralf" last="Altmeyer">Ralf Altmeyer</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-55925" status="VALID">          <orgName>Centre de recherche Université de Hong-Kong-Pasteur</orgName>
          <desc>            <address>              <addrLine>Hong Kong University Pasteur Research Centre - 1/F Dexter HC Man Building 8, Sassoon Road Pokfulam</addrLine>
              <country key="HK"></country>
            </address>
            <ref type="url">http://www.hkupasteur.hku.hk/</ref>
          </desc>
          <listRelation>            <relation active="#struct-301247" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-301247" type="direct"><org type="regroupinstitution" xml:id="struct-301247" status="VALID">          <orgName>Réseau International des Instituts Pasteur</orgName>
          <orgName type="acronym">RIIP</orgName>
          <desc>            <address>              <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">https://research.pasteur.fr/ip-network</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Hong Kong</country>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1016/j.jcv.2007.02.005</idno>
<series><title level="j">Journal of Clinical Virology</title>
<idno type="ISSN">1386-6532</idno>
<imprint><date type="datePub">2007-05</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>BACKGROUND: Highly pathogenic avian influenza (HPAI) H5N1 has spread globally in birds and infected over 270 humans with an apparently high mortality rate. Serologic studies to determine the extent of asymptomatic H5N1 infection in humans and other mammals and to investigate the immunogenicity of current H5N1 vaccine candidates have been hampered by the biosafety requirements needed for H5N1 micro-neutralization tests. OBJECTIVE: Development of a serodiagnostic tool for highly pathogenic influenza that reproduces H5N1 biology but can be used with less biohazard. STUDY DESIGN: We have generated and evaluated H5 hemagglutinin pseudotyped lentiviral particles encoding the luciferase reporter (H5pp). RESULTS: H5pp entry into target cells depends on alpha2-3 cell surface sialic acids and requires low pH for membrane fusion. H5pp infectivity is specifically neutralized by sera from patients and animals infected with H5N1 and correlates well with conventional microneutralization test. CONCLUSIONS: H5pp reproduce H5N1 influenza virus entry into target cells and potentially provides a high-throughput and safe method for sero-epidemiology.</p>
            </div>
</front>
</TEI>
<affiliations><list><country><li>Cambodge</li>
<li>Hong Kong</li>
<li>République populaire de Chine</li>
</country>
</list>
<tree><country name="Hong Kong"><noRegion><name sortKey="Nefkens, Isabelle" sort="Nefkens, Isabelle" uniqKey="Nefkens I" first="Isabelle" last="Nefkens">Isabelle Nefkens</name>
</noRegion>
<name sortKey="Altmeyer, Ralf" sort="Altmeyer, Ralf" uniqKey="Altmeyer R" first="Ralf" last="Altmeyer">Ralf Altmeyer</name>
<name sortKey="Garcia, Jean Michel" sort="Garcia, Jean Michel" uniqKey="Garcia J" first="Jean-Michel" last="Garcia">Jean-Michel Garcia</name>
<name sortKey="Lagarde, Nadege" sort="Lagarde, Nadege" uniqKey="Lagarde N" first="Nadège" last="Lagarde">Nadège Lagarde</name>
<name sortKey="Ling, Chu Shui" sort="Ling, Chu Shui" uniqKey="Ling C" first="Chu Shui" last="Ling">Chu Shui Ling</name>
<name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name>
<name sortKey="Tang, Dong Jiang" sort="Tang, Dong Jiang" uniqKey="Tang D" first="Dong Jiang" last="Tang">Dong Jiang Tang</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Nicholls, John" sort="Nicholls, John" uniqKey="Nicholls J" first="John" last="Nicholls">John Nicholls</name>
</noRegion>
</country>
<country name="Cambodge"><noRegion><name sortKey="Buchy, Philippe" sort="Buchy, Philippe" uniqKey="Buchy P" first="Philippe" last="Buchy">Philippe Buchy</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

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Hemagglutinin pseudotyped lentiviral particles: characterization of a new method for avian H5N1 influenza sero-diagnosis.

Hemagglutinin pseudotyped lentiviral particles: characterization of a new method for avian H5N1 influenza sero-diagnosis.

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